Acoustic tweezing spectroscopy (ATS) enables the measurement of changes in the shear viscoelasticity of blood during coagulation. Using samples from healthy volunteers, hemophilia patients, and smokers, we demonstrated that ATS viscosity data can accurately determine prothrombin time (PT) and activated partial thromboplastin time (aPTT), while ATS elasticity data correlates with fibrinogen concentration. Thus, ATS can measure PT, aPTT, and fibrinogen in whole blood samples or hemolyzed plasma samples, which were previously discarded for coagulation analysis due to hemoglobin interfering with photo-optical measurements. This study, led by Nithya Kasireddy, has been published in Analytical and Bioanalytical Chemistry.
