FAQ
The cuvette technology requires around 0.7 ml -2.5 ml of sample, on the lower end for non-dialysis applications of the technology. For large molecules/colloids >10^6 g/mol, concentration can be as low as 0.020 mg/ml, around 50 micrograms per experiment. For smaller molecules, ~10,000 g/mol to as much as 1 mg or more may be needed per experiment. The technology encompasses a wide span of concentration ranges, from 0.001 mg/ml to 200 mg/ml.
Yes, the cuvette technology will integrate seamlessly with any of your analytical instrumentation that accepts a standard 1 cm cuvette (light scattering, UV/vis, Fluorimetry, CD, etc.), no modifications needed. A specialized cap is provided to seal the cuvette with the device attached. The data will be collected and can be observed via the analytical instrumentation’s current platform.
The cuvette-based technology’s unique continuous flow enables an efficient alternative method to traditional formulation techniques, such as chromatography or screening platforms, which depend on separately prepared, isolated samples for each excipient, buffer, or dilution condition. Rather than analyzing numerous discrete, static samples, the cuvette technology's continuous gradient allows users to observe a wide range of formulation concentrations with one sweep, offering more comprehensive data with less sample prep.
Unlike other plate or probe based systems on the market, our spectroscopic technology utilizes standard 1cm cuvettes to enable real-time monitoring of biologics under dynamic conditions. While other systems may require fixed samples and specific software, our design integrates seamlessly with existing analytical instruments. The automated continuous mixing capability and dialysis feature allow users to study biologic stability and aggregation under a continuous concentration gradient, eliminating the need for discrete sample prep and testing such as with chromatography. This provides researchers with a comprehensive, flexible, and easily adaptable approach to understanding stability, reversibility, and aggregation behavior.
While it can serve as a standalone alternative for formulation studies and aggregation analysis, it also complements existing systems by filling in key gaps, such as kinetic behavior under changing conditions, reversibility, and design space. The technology can complement current high throughput methods for candidate selection by subsequently determining specific stable concentration ranges.